Keywords
metal resistant bacteria
soil
t-DNA.
How to Cite
Abstract
The study was performed to show how industrial activity affected soil quality in terms of soil DNA quality and quantity as well as soil characteristics. Soil material originated from an urban area of the Silesia Region (SW Poland). The soil characteristics were estimated: texture, moisture, pH, redox potential (Eh), and total carbon content (TOC), followed by determination of selected heavy metals (Pb, Cd, Zn, Cr, Fe, Cu). The last step was the isolation of soil DNA, its concentration and identification of microorganisms. The results showed that although the studied soil was heavily contaminated with heavy metals, there were still some metal-resistant microorganisms able to sustain soil activity. Moreover, these organisms are not present in the NCBI database, which encourages further studies aimed at identification of new organisms that may be useful in research of metal resistance as well as soil reclamation and remediation.References
Roesch LF, Fulthorpe RR, Riva A, Casella G, Hadwin AK, Kent AD, Daroub SH, et al. Pyrosequencing enumerates and contrasts soil microbial diversity. ISME J 2007; 1: 283-290.
Wolińska A, Stępniewska Z, Wołoszyn A, Pytlak A, Dziuba A. Means of molecular nucleic acids analysis in soil investigations. Acta Agrop 2011; 194: 1-62.
Anyanwu CU, Nwankwo SC, Moneke AN. Soil bacteria response to introduced metal stress. Int J Bas Appl Sci 2011; 11: 73-76.
Harichova J, Karelova E, Pangallo D, Ferianc P. Structure analysis of bacterial community and their heavy metal resistance determinants in the heavy metal contaminated soil sample. Biologia 2012; 67: 1038-1048. http://dx.doi.org/10.2478/s11756-012-0123-9
Genthe B, Le Roux WJ, Schachtschneider K, Oberholster PJ, Aneck-Hahn NH, Chamier J. Health risk implications from simultaneous exposure to multiple environmental contaminants. Ecotoxicol Environ Saf 2013; 93: 171-179. http://dx.doi.org/10.1016/j.ecoenv.2013.03.032
Hou D, He J, Lü C, Ren L, Fan Q, Wang J, Xie Z. Distribution characteristic and potential ecological risk assessment of heavy metals (Cu, Pb, Zn, Cd) in water and sediments from Lake Dalinouer, China. Ecotoxicol Environ Saf 2013; 93: 135-144. http://dx.doi.org/10.1016/j.ecoenv.2013.03.012
Kozdroj J, van Elsas JD. Structural diversity of microorganisms in chemically perturbated soil assessed by molecular and cytochemical approaches. J Microbiol Met 2001; 43: 197-212. http://dx.doi.org/10.1016/S0167-7012(00)00197-4
Chien C, Kuo Y, Chen C, Yeh C, Yeh W. Microbial diversity of soil bacteria in agricultural field contaminated with heavy metals. J Environ Sci 2008; 20: 359-363. http://dx.doi.org/10.1016/S1001-0742(08)60056-X
Wu H, Zhao H, Wen C, Guo J, Xu M, Li X. A comparative study of bacterial community structures in the sediments from brominated flame retardants contaminated river and noncontaminated reservoir. Afr J Microbiol Res 2012; 6: 3248-3260.
Zhuang J, McCarthy JF, Perfect E, Mayer LM, Jastrow JD. Soil water hysteresis in water-stable microaggregates as affected by organic matter. Soil Sci Soc Am J 2008; 72: 212-220. http://dx.doi.org/10.2136/sssaj2007.0001S6
Wolińska A, Stępniewska Z, Wołoszyn A, Rzewuska M. Interrelations between DNA concentration and physicochemical parameters in the less soil profile. Acta Agrop 2012; 19: 437-448.
Polish Regulation for Industrial Areas. Dz U 2002; No 165/1359 (in Polish).
Ciarkowska K, Sołek-Podwika K, Wieczorek J. Enzyme activity as an indicator of soil rehabilitation process at a zincand lead ore mining and processing area. J Environ Manag 2014; 132: 250-256. http://dx.doi.org/10.1016/j.jenvman.2013.10.022
Acosta-Martinez V, Burow G, Zobeck TM, Allen VG. Soil microbial communities and function in alternative systems to continuous cotton. Soil Sci Soc Am J 2010; 74: 1181-1192. http://dx.doi.org/10.2136/sssaj2008.0065
Stępniewska Z, Wolińska A, Ziomek J. Response of soil catalase activity to chromium contamination. J Environ Sci 2009; 21: 1142-1147. http://dx.doi.org/10.1016/S1001-0742(08)62394-3
Pan J, Yu L. Effects of Cd and Pb on soil enzyme activities and microbial community structure. Ecol Eng 2011; 37: 1889-1894. http://dx.doi.org/10.1016/j.ecoleng.2011.07.002
Wolińska A, Stępniewska Z. Dehydrogenases in the soil environment. In: Cantuto R, Ed. Dehydrogenases. Rijeka: In-Tech Publisher 2012; pp. 183-210. http://dx.doi.org/10.5772/48294
Xie W, Zhou J, Wang H, Chen X, Lu Z, Yu J, Chen X. Shortterm effects of copper, cadmium and cypermetehrin on dehydrogenase activity and microbial functional diversity in soils after long-term mineral and organic fertilization. Agric Ecosyst Environ 2009; 149: 450-456. http://dx.doi.org/10.1016/j.agee.2008.10.021
Abdelatey LM, Khalil KBK, Ali TH, Mahrous KF. Heavy metal resistance and gene expression analysis of metal resistance gene in gram positive and gram negative bacteria present in Egyptian soil. J Appl Sci Environ Sanit 2011; 6: 201-211.
Gomes J, Steiner W. Extermophiles and extermozymes. Food Technol Biotechnol 2004; 42: 223-235.
Li D, Hendry P, Faiz M. A survey of the microbial populations in some Australian coalbed methane reservoirs. Int J Coal Geol 2008; 76: 14-24. http://dx.doi.org/10.1016/j.coal.2008.04.007
Belimov AA, Hontzeas N, Safronova VI, Demchinskaya SV, Piluzza G, Bullitta S, et al. Cadmium-tolerant plant growthpromoting bacteria associated with the roots of Indian mustard (Brassica juncea L. Czern). Soil Biol Biochem 2005; 37: 241-250. http://dx.doi.org/10.1016/j.soilbio.2004.07.033
Van Houdt R, Toussaint A, Ryan MP, Pembroke JT, Mergeay M, Adley CC. The Tn4371 Ice family of bacterial mobile genetic elements. In: Roberts AP, Mullany P, Eds. Bacterial Integrative Mobile Elements. Austin: Landes Biosciences 2013; pp. 179-200.
Chaudhuri SK, Lovley DR. Electricity generation by direct oxidation of glucose in mediatorless microbial fuel cell. Nat Biotech 2003; 21: 1229-1232. http://dx.doi.org/10.1038/nbt867
Jiang K, Sanseverino J, Chauhan A, Lucas S, Copeland A, Lapidus A, et al. Complete genome sequence of Thaurea aminoaromatica strain MZ1T. Standards Genom Sci 2012; 6: 325-335. http://dx.doi.org/10.4056/sigs.2696029
Mahvi AH, Diels L. Biological removal of cadmium by Alacaligenes eutrophus CH34. Int J Environ Sci Tech 2004; 1: 199-204. http://dx.doi.org/10.1007/BF03325833
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